Our collaborators at the Biozentrum integrated single-cell lysis with mass spectrometry and reverse-phase protein arrays, enabling correlative single- and few-cell analyses that combine light microscopy with metabolomics and targeted proteomics.
Originally, CryoWriter technology was developed for single-cell analysis via electron microscopy (single-cell visual proteomics), with its writing system directly coupled to a single-cell lysis method for adherent eukaryotic cells. Now, the system integrates light microscopy, cell targeting, and lysis with liquid chromatography-mass spectrometry for single-cell metabolite detection and protein microarrays for protein analysis.
These technologies paves the way for correlative multiomics analysis on the single cell level!
Relevant publications for single-cell visual proteomics:
- Syntychaki A, Rima L, Schmidli C, Stohler T, Bieri A, Sütterlin R, et al. `Differential visual proteomics’: Enabling the proteome-wide comparison of protein structures of single-cells. J Proteome Res. 2019;18(9):3521–31.
- Arnold SA, Albiez S, Opara N, Chami M, Schmidli C, Bieri A, et al. Total sample conditioning and preparation of nanoliter volumes for electron microscopy. ACS Nano. 2016;10(5):4981–8.
- Kemmerling S, Arnold SA, Bircher BA, Sauter N, Escobedo C, Dernick G, et al. Single-cell lysis for visual analysis by electron microscopy. J Struct Biol. 2013;183:467–73.
