Faraday Discussion: Coverslip injector

cryo electron micrograph with standard preparation — Chaperonin 60 was prepared using cryoWriter technology without and with the coverslip injector module. Scalebar: 200 nm.

cryo electron micrograph after modulation of the air-water interface — Chaperonin 60 was prepared using cryoWriter technology without and with the coverslip injector module. Scalebar: 200 nm.

Together with cryoWrite and Roche, a research group in the Biozentrum of the University Basel presented a Faraday Discussion paper improving the grid quality and reducing background noise using cryoWriter technology. They use a “coverslip” injector to modulate the air-water interface with amphiphilic molecules:

Rima, L.; Zimmermann, M.; Fränkl, A.; Clairfeuille, T.; Lauer, M.; Engel, A.; Engel, H.-A.; Braun, T. CryoWriter: A Blotting Free Cryo-EM Preparation System with a Climate Jet and Cover-Slip Injector. Faraday Discussions2022. https://doi.org/10.1039/D2FD00066K.