Cryo-EM methods made impressive progress during the last years. New detectors and data processing algorithms advanced the resolution of single particle analysis significantly. This single-particle approach allows structure determination of proteins without crystallization and replaces X-ray methods, particularly for large protein complexes.
Structure determination of biological molecules has been dominated by X-Ray analysis of three-dimensional crystals. In the last decade, cryo-EM evolved to the structural biologists driving method, as indicated by the exponential growth of atomic structures solved by the single-particle analysis approach.
However, one bottle-neck remained: Sample preparation. cryoWrite provides unique microfluidic methods for protein isolation, sample preparation, and nano-analytics to overcome this remaining hurdle. This may contribute to a further growth of cryo-EM for protein structure determination.
Last update: 2020-12-01
